Effect of a low-intensity electromagnetic field on the sensitivity of crustaceans Daphnia magna Straus (Daphniidae, Crustacea) to heavy metal salts on the example of potassium bichromate and cadmium sulfate

The influence of low-intensity electromagnetic fields (EMF) on the sensitivity of Daphnia magna Straus, 1820 to two model toxicants, namely, potassium dichromate (K2Cr2O7) and cadmium sulfate (3CdSO4ꞏ8H2O), was investigated. Two EMF exposure modes with a frequency of 30 MHz were used, namely: with a constant amplitude and an amplitude modulation (AM) mode with a frequency of 50 Hz. The EMF effect on the sensitivity of Daphnia was assessed by observing changes in the survival of the crustaceans placed into toxicant solutions after EMF ex- posure. Differences in the sensitivity of Daphnia exposed to EMF to the studied toxicants were manifested in the dynamics of survival reduction in salt solutions over a 96-hour exposure period. The most noticeable differences between the effects of cadmium and dichromate on the crustaceans were observed in the AM mode. To analyze the inheritance of traits, a comparison was made of the effects of heavy metals on the survival of offspring from directly exposed females (series I) and the unexposed offspring of exposed females (series II). The offspring of Daphnia from exposed females (series II) exhibited higher resistance to еру toxicants compared to series I. To explain the increased resistance of the offspring from exposed crustaceans, the possibility of adaptive mechanisms being triggered in response to the damaging informational impact of EMF on the genetic material of the females is discussed.

1. Bertin G., Averbeck D. Cadmium: Cellular effects, modifications of bio-molecules, modulation of DNA repair and genotoxic consequences (a review). Biochimie, 2006, vol. 88, iss. 11, pp. 1549–1559. https://doi.org/10.1016/j.biochi.2006.10.

2. Binhi V. N., Rubin A. B. Theoretical concepts in magnetobiology after 40 years of research. Cells, 2022, vol. 11, iss. 2, article no. 274. https://doi.org/10.3390/cells11020274

3. Binhi V., Rubin A.B. On the quantum nature of magnetic phenomena in biology. Physics of Biology and Medicine, 2023, no. 1, pp. 44–73 (in Russian). https://doi.org/10.7256/27300560.2023.1.40435

4. Filenko O. F., Mikheeva I. V. Principles of Aquatic Toxicology. Moscow, Kolos, 2007. 144 p. (in Russian).

5. Gapochka L. D., Gapochka M. G., Drozhzhina T. S., Shavyrina O. B. A hidden effect of irradiation in experiments with microalgae. In: Autotrophic Microorganisms: Proceedings of the 4th All-Russian Symposium with international participation. Moscow, MAX Press, 2010, pp. 32–33 (in Russian).

6. Gapochka L. D., Gapochka M. G., Drozhzhina T. S., Isakova E. F., Pavlova A. S., Shavyrina O. B. Effects of irradiation by the low-intensity electromagnetic field of the millimetric range on the Daphnia magna culture at various developmental stages. Moscow University Biological Sciences Bulletin, 2012, vol. 67, iss. 2, pp. 82–87. https://doi.org/10.3103/S009639251202006X

7. Garkavi L. Kh., Kvakina E. B., Shikhlyarova A. I., Kuz'menko T. S., Barsukov L. P., Mar'yanovskaya G. Ya., Sheiko Ye. A., Yevstratova O. F., Zhukova G. V. Magnetic fields, adaptional reactions and the self-organization of living system. Biophysics, 1996, vol. 41, no. 4, pp. 909–916.

8. Gopi R. A., Ayyappan S., Chandrasehar G., Krishna V., Goparaju A. Effect of potassium dichromate on the survival and reproduction of Daphnia magna. Bulletin of Environment Pharmacology and Life Sciences, 2012, vol. 1, iss. 7, pp. 89–94.

9. Hammer Ø., Harper D. A. T., Ryan P. D. Past: Paleontological Statistics Software Package for education and data analysis. Palaeontologia Electronica, 2001, vol. 4, iss. 1, article no. 4.

10. Hoeijmakers J. H. J. Genome maintenance mechanisms for preventing cancer. Nature, 2001, vol. 411, no. 6835, pp. 366–374. https://doi.org/10.1038/35077232

11. Krylov V. V. Direct and prolonged effects of the action of low frequency alternating electromagnetic fields on reproduction parameters of Daphnia magna. Hydrobiological Journal, 2007, vol. 43, no. 6, pp. 71–82. https://doi.org/10.1615/HydrobJ.v43.i6.60

12. Martins J., Teles L. O., Vasconcelos V. Assays with Daphnia magna and Danio rerio as alert systems in aquatic toxicology. Environment International, 2007, vol. 33, no. 3, pp. 414–425.

13. McRee D. I. Review of Soviet/Eastern European research on health aspects of microwave radiation. Bulletin of the New York Academy of Medicine, 1979, vol. 55, iss. 11, pp. 1133–1151.

14. Medyankina M. V., Philenko O. Ph., Shirokov D. A. Influence of bottom sediments at the toxicity of heavy metals for daphnia. 1. Chrome. Environmental Systems and Devices, 2006, no. 12, pp. 39–42 (in Russian).

15. Methodological Guidelines for the Development of Water Quality Standards for Water Bodies of Fishery Significance, Including Standards for Maximum Permissible Concentrations of Harmful Substances in the Waters of Water Bodies of Fishery Significance. Approved by the Order of the Federal Agency for Fishery from August 4, 2009 No. 695. Moscow, Federal Agency for Fishery, 2009. 120 p. (in Russian).

16. Methodology for Determination of Toxicity of Water and Water Extracts from Soils, Sewage Sludge, Wastes by Mortality and Change in Fecundity of Daphnia: FR.1.39.2007.03222. Moscow, Aquaros, 2007. 51 p. (in Russian).

17. Methodology of Measuring the Mass Concentration of Cadmium Ions in Natural and Waste Waters by Photometric Method with Dithizone. PND F 14.1:2.45-96. Moscow, Federal Center for Analysis and Assessment of Technogenic Impacts Publ., 2013. 21 p. (in Russian).

18. Methodology for Measuring the Mass Concentration of Chromium Ions in Drinking, Natural and Waste Waters by the Photometric Method with Diphenyl-Carbazide. PNDF 14-1:2:4.52-96. Moscow, Federal Center for Analysis and Assessment of Technogenic Impacts Publ., 2016. 22 p. (in Russian).

19. Papoyan G. K., Filenko O. F., Yusupov V. I., Vorobyeva О. V., Zotov K. V., Bagratashvilli V. N. Effect of a low-intensity 30 MHz electromagnetic field on Daphnia magna Straus (Daphniidae, Crustacea) crustaceans of various ages. Povolzhskiy Journal of Ecology, 2017, no. 3, pp. 314–320 (in Russian).

20. Papoyan G. K., Filenko O. F., Yusupov V. I., Zotov K. V. Influence of electromagnetic radiation on the sensitivity of freshwater crustacean Daphnia magna, Straus to cadmium sulfate. Environmental Systems and Devices, 2018a, no. 9, pp. 11–17 (in Russian).

21. Papoyan G. K., Filenko O. F., Yusupov V. I., Vorob’yeva O. V., Zotov K. V., Bagratashvili V. N. Influence of low-intensity electromagnetic field on some biological parameters of freshwater crustaceans Daphnia magna Straus. Inland Water Biology, 2018b, vol. 11, iss. 2, pp. 124– 128. https://doi.org/10.1134/S1995082918020141

22. Pryakhin E. A., Akleyev A. V. Electromagnetic Fields and Biological Systems: Stress and Adaptation. Chelyabinsk, Polygraph-Master, 2011. 239 p. (in Russian).

23. Sigel A., Sigel H., Sigel R., eds. Metal Ions in Biological Systems. Vol. 43. Biogeochemical Cycles of Elements. Boca Raton, CRC Press, 2005. 352 p.

24. Stroganov N. S., Putintsev A. I., Isakova E. F., Shigin V. I. Method of toxicological control of wastewater. Biologicheskie nauki, 1979, no. 2, pp. 90–96 (in Russian).

25. Testing of Chemicals of Environmental Hazard. Daphnia sp. Acute Immobilisation Test. GOST 32536-2013. Moscow, Standartinform, 2014. 10 p. (in Russian).

26. Vijayalaxmi B. Z., Frei M. R., Dusch S. J., Guel V., Meltz M. L., Jauchem J. R. Frequency of micronuclei in the peripheral. blood и bone marrow of cancer-prone mice chronically exposed to 2450 MHz radiofrequency radiation. Radiation Research, 1997, vol. 147, no. 4, pp. 495–500.

27. Vijayalaxmi B. Z., Obe G. Controversial cytogenetic observations in mammalian somatic cells exposed to radiofrequency radiation. Radiation Research, 2004, vol. 162, no. 5, pp. 481–496. https://doi.org/10.1667/rr3252

28. Vodyanitsky Y. N. Heavy Metals and Metalloids in Soils. Moscow, В. V. Dokuchaev Soil Institute of Russian Academy of Agricultural Sciences Publ., 2008. 164 p. (in Russian).