Leukocyte blood composition of Emys orbicularis and Mauremys capsica (Reptilia: Testudines: Emydidae, Geoemydidae) at syntopy
https://doi.org/10.35885/1684-7318-2022-1-79-93
Abstract
Data on the leukocyte blood composition of two co-habiting species of freshwater turtles, Emys orbicularis and Mauremys caspica, from the Republic of Dagestan (Russia) are provided. Lymphocytes are the predominant cells in the blood of females and males of both species. Heterophils predominated among granulocytes. Two-way multivariate analysis of variance showed a statistical significance of the influence of two factors on the leukocyte formula of freshwater turtles, namely: species (λWilks = 0.459, p < 0.001), sex (λWilks = 0.809, p = 0.019) and their interaction (λWilks < 0.001, p < 0.001). The blood formula (WBC) of E. orbicularis females showed higher levels of eosinophils (z = 3.040, p = 0.001) and basophils (z = 5.394, p < 0.001) and reduced levels of heterophils (z = 3.579, p < 0.001) and lymphocytes (z = 3.391, p < 0.001) compared to M. caspica females. Males of E. orbicularis differed from those of M. caspica by increased levels of eosinophils (z = 2.716, p = 0.003) and basophils (z = 4.829, p < 0.001) and a reduced level of heterophils (z = 3.474, p < 0.001). With general adaptive responses, interspecific differences were clearly expressed: the variability in the parameters of the white blood cell system of E. orbicularis was associated with rearrangement of the ratio of granulocyte cells (heterophils, eosinophils and basophils). The predominance of a specific component of the immune system (lymphocytes) was found in the immune responses of M. caspica. Changes in the leukocyte profiles of freshwater turtles were associated with a change in the agranulocyte–granulocyte ratio, which was confirmed by statistically significant differences in the integral leukocyte indices. The leukocyte profile of Emys orbicularis testified to the insufficient resource of adaptive mechanisms in specific environmental conditions. On the other hand, the blood formula (WBC) showed the possibility of an active response, more pronounced in M. caspica than in E. orbicularis.
About the Authors
E. B. Romanova
Lobachevsky State University of Nizhni Novgorod
Russian Federation
Russian Federation
Elena B. Romanova
23 Gagarin Avenue, Nizhni Novgorod 603950
I. A. Stolyarova
Lobachevsky State University of Nizhni Novgorod
Russian Federation
Russian Federation
Irina A. Stolyarova
23 Gagarin Avenue, Nizhni Novgorod 603950
A. G. Bakiev
Samara Federal Research Center of RAS, Institute of Ecology of the Volga River Basin of Russian Academy of Sciences
Russian Federation
Russian Federation
Andrey G. Bakiev
10 Komzina St., Togliatti 445003
R. A. Gorelov
Samara Federal Research Center of RAS, Institute of Ecology of the Volga River Basin of Russian Academy of Sciences
Russian Federation
Russian Federation
Roman A. Gorelov
10 Komzina St., Togliatti 445003
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Review
For citations:
Romanova E.B., Stolyarova I.A., Bakiev A.G., Gorelov R.A. Leukocyte blood composition of Emys orbicularis and Mauremys capsica (Reptilia: Testudines: Emydidae, Geoemydidae) at syntopy. Povolzhskiy Journal of Ecology. 2022;(1):79-93. (In Russ.) https://doi.org/10.35885/1684-7318-2022-1-79-93
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