sevinПОВОЛЖСКИЙ ЭКОЛОГИЧЕСКИЙ ЖУРНАЛPovolzhskiy Journal of Ecology1684-73182541-8963Федеральное государственное бюджетное учреждение науки Институт проблем экологии и эволюции им. А. Н. Северцова РАН; Товарищество научных изданий КМК.10.35885/1684-7318-2025-2-153-164sevin-654Research ArticleСтатьиСравнительная характеристика генетического полиморфизма популяций ротана Perccottus glenii (Perciformes, Odontobutidae) Волжского и Иртышского бассейновComparative characteristics of the genetic polymorphism of populations of Chinese sleeper Perccottus glenii (Perciformes, Odontobutidae) in the Volga and Irtysh basins.https://orcid.org/0000-0002-3782-3014ЖигилеваО. Н.ZhigilevaO. N.

Жигилева Оксана Николаевна, Кафедра экологии и генетики Института биологии

625003, г. Тюмень, ул. Володарского, д. 6

6 Volodarskogo St., Tyumen 625003

https://orcid.org/0009-0008-7574-2734КолесниковИ. П.KolesnikovI. P.

Колесников Илья Павлович, Кафедра экологии и генетики Института биологии

625003, г. Тюмень, ул. Володарского, д. 6

6 Volodarskogo St., Tyumen 625003

https://orcid.org/0000-0002-2595-7453ФайзулинА. И.FayzulinA. I.

Файзулин Александр Ильдусович

10 Komzina St., Togliatti 445003

https://orcid.org/0000-0001-9932-6227РубановаМ. В.RubanovaM. V.

Рубанова Марина Васильевна

10 Komzina St., Togliatti 445003

Тюменский государственный университетРоссияTyumen State UniversityRussian FederationСамарский федеральный исследовательский центр РАН, Институт экологии Волжского бассейна РАН РоссияРоссияSamara Federal Research Center of Russian Academy of Sciences, Institute of Ecology of the Volga River Basin of Russian Academy of SciencesRussian Federation20253107202502153164Copyright © Жигилева О.Н., Колесников И.П., Файзулин А.И., Рубанова М.В., 20252025Жигилева О.Н., Колесников И.П., Файзулин А.И., Рубанова М.В.Zhigileva O.N., Kolesnikov I.P., Fayzulin A.I., Rubanova M.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sevin.elpub.ru/jour/article/view/654

Даны оценки генетического разнообразия и дифференциации популяций ротана Perccottus glenii из водоемов Волжского и Иртышского бассейнов на основании ISSR маркеров. В изученной части Волжского бассейна доля полиморфных локусов (Р) ротана составила 80.4%, генетическое разнообразие (h) – 0.26. Все исследованные популяции ротана в районе Куйбышевского водохранилища (Ульяновская, Самарская область, Тольятти) генетически слабо дифференцированы и происходят из одного источника инвазии. Показатели полиморфизма и генетического разнообразия ротана в водоемах бассейна р. Иртыш выше, чем в Поволжье, составляют: Р = 98.2%, h = 0.33. Генетическая дифференциация между популяциями оз. Ипкуль и р. Тобол (Тюменская область) слабо выражена (Gst = 0.07), поток генов между ними – значительный, что свидетельствует об их недавнем расхождении или происхождении одной популяции от другой. Между популяциями оз. Березово (Курганская область) и оз. Малое Плоское (Казахстан) генетическая дифференциация высокая (GST = 0.31), что может свидетельствовать о разных источниках инвазии ротана на юге Сибири и в Северном Казахстане. Данные о более высоком полиморфизме ротана в бассейне р. Иртыш, а также значительной генетической дифференциации между популяциями ротана Волжского и Иртышского бассейнов (GST = 0.40) лишь частично подтверждают общепринятую гипотезу о заселении ротаном Сибири через Южно-Уральский инвазионный коридор, указывая на наличие нескольких разнородных источников инвазии ротана в ОбьИртышском бассейне. 

The level of genetic diversity and differentiation of populations of the Chinese sleeper Perccottus glenii from water bodies of the Volga and Irtysh basins was assessed based on ISSR markers. In the studied part of the Volga basin, the proportion of polymorphic loci (P) of the Chinese sleeper was 80.4%, genetic diversity (h) was 0.26. All studied populations of the Chinese sleeper from the Kuibyshev Reservoir area (Ulyanovsk, Samara region, Togliatti) were genetically weakly differentiated and originate from a single source of invasion. The polymorphism and genetic diversity of the Chinese sleeper in the Irtysh basin were higher than in the Volga region and amounted to: P = 98.2%, h = 0.33. Genetic differentiation between the populations of the Ipkul Lake and the Tobol River (Tyumen region) was weakly expressed (GST = 0.07), the gene flow between them was significant, which indicates their recent divergence or the origin of one population from another. The genetic differentiation between the populations of Lake Berezovo (Kurgan region) and Lake Maloe Ploskoe (Kazakhstan) was high (GST = 0.31), which may indicate different sources of invasion of the Chinese sleeper in southern Siberia and northern Kazakhstan. Data on the higher polymorphism of the Chinese sleeper in the Irtysh River basin, as well as significant genetic differentiation between its populations in the Volga and Irtysh basins (GST = 0.40), only partially confirm the generally accepted hypothesis of the colonization of Siberia by the Chinese sleeper through the South Ural invasion corridor, indicating the presence of several heterogeneous invasion sources in the Ob–Irtysh basin. 

ротанбиологические инвазииполиморфизмДНК-маркерыпути расселенияChinese sleeperbiological invasionspolymorphismDNA markersentry routesИсследование было выполнено при финансовой поддержке Правительства Тюменской области в рамках проекта Западно-Сибирского межрегионального научнообразовательного центра (№ 89-ДОН (2)) и по теме государственного задания Института экологии Волжского бассейна РАН – филиала Самарского научного центра РАН (№ 1021060107212-5-1.6.20; 1.6.19 «Изменение, устойчивость и сохранение биологического разнообразия под воздействием глобальных изменений климата и интенсивной антропогенной нагрузки на экосистемы Волжского бассейна»).This work was performed with the support of the Government of the Tyumen Region within the framework of the project of the West Siberian Interregional Scientific and Educational Center (No. 89-DON (2)) and the State Theme of the Institute of Ecology of the Volga Basin of the Russian Academy of Sciences – branch of the Samara Scientific Center of the Russian Academy of Sciences (No. 1021060107212-5-1.6.20; 1.6.19 “Change, Sustainability and Conservation of Biological Diversity Under the Influence of Global Climate Change and Intensive Anthropogenic Load on the Ecosystems of the Volga Basin”).ReferencesAlyamkin G. V., Zhigileva O. N., Zhokhov A. E. Genetic variability of the Amur sleeper Perccottus glenii and of their parasite, cestode Nippotaenia mogurndae, outside the natural area of distribution. Inland Water Biology, 2022, vol. 15, no. 2, pp. 179–188. https://doi.org/10.1134/ S1995082922010023Alyamkin G. V., Zhigileva O. N., Zhokhov A. E. Genetic variability of the Amur sleeper Perccottus glenii and of their parasite, cestode Nippotaenia mogurndae, outside the natural area of distribution. Inland Water Biology, 2022, vol. 15, no. 2, pp. 179–188. https://doi.org/10.1134/ S1995082922010023Artaev O. N. Perccottus glehni in the Moksha river basin: Distribution and preferred groups of water bodies. Proceedings of the Mordovia State Nature Reserve, 2016, iss. 17, pp. 3–7 (in Russian).Artaev O. N. Perccottus glehni in the Moksha river basin: Distribution and preferred groups of water bodies. Proceedings of the Mordovia State Nature Reserve, 2016, iss. 17, pp. 3–7 (in Russian).Baranov V. Y., Vasil’ev A. G. Phenotypic plasticity of the Amur sleeper (Perccottus glenii) invasive populations during the colonization of water bodies. Russian Journal of Biological Invasions, 2022, vol. 13, iss. 4, pp. 412–427. https://doi.org/10.1134/S2075111722040038Baranov V. Y., Vasil’ev A. G. Phenotypic plasticity of the Amur sleeper (Perccottus glenii) invasive populations during the colonization of water bodies. Russian Journal of Biological Invasions, 2022, vol. 13, iss. 4, pp. 412–427. https://doi.org/10.1134/S2075111722040038Borisova A. T. Accidental invaders into the water bodies of Uzbekistan. Journal of Ichthyology, 1972, vol. 12, no. 1, pp. 49–53 (in Russian).Borisova A. T. Accidental invaders into the water bodies of Uzbekistan. Journal of Ichthyology, 1972, vol. 12, no. 1, pp. 49–53 (in Russian).Chemagin A. A. Distribution of Amur-sleeper (Perccottus glenii Dybowski, 1877) in floodplain lakes of the Lower Irtysh. Fundamental Research, 2014, no. 11, pp. 2656–2660 (in Russian).Chemagin A. A. Distribution of Amur-sleeper (Perccottus glenii Dybowski, 1877) in floodplain lakes of the Lower Irtysh. Fundamental Research, 2014, no. 11, pp. 2656–2660 (in Russian).Doherty T. S., Glen A. S., Nimmo D. G., Ritchie E. G., Dickman C. R. Invasive predators and global biodiversity loss. Proceedings of the National Academy of Sciences, 2016, vol. 113, no. 40, pp. 11261–11265. https://doi.org/10.1073/pnas.1602480113Doherty T. S., Glen A. S., Nimmo D. G., Ritchie E. G., Dickman C. R. Invasive predators and global biodiversity loss. Proceedings of the National Academy of Sciences, 2016, vol. 113, no. 40, pp. 11261–11265. https://doi.org/10.1073/pnas.1602480113Evlanov I. A., Kozlovskij S. V., Antonov P. I. Kadastr ryb Samarskoj oblasti [Cadastre of Fish in the Samara Region]. Togliatti, Institute of Ecology of the Volga River Basin of Russian Academy of Sciences Publ., 1998. 222 p. (in Russian).Evlanov I. A., Kozlovskij S. V., Antonov P. I. Kadastr ryb Samarskoj oblasti [Cadastre of Fish in the Samara Region]. Togliatti, Institute of Ecology of the Volga River Basin of Russian Academy of Sciences Publ., 1998. 222 p. (in Russian).Grabowska J., Kvach Yu., Rewicz T., Pupins M., Kutsokon I., Dykyy I., Antal L., Zieba G., Rakauskas V., Trichkova T., Ceirans A., Grabowski M. First insights into the molecular population structure and origins of the invasive Chinese sleeper, Perccottus glenii, in Europe. NeoBiota, 2020, vol. 57, pp. 87–107. https://doi.org/10.3897/neobiota.57.48958Grabowska J., Kvach Yu., Rewicz T., Pupins M., Kutsokon I., Dykyy I., Antal L., Zieba G., Rakauskas V., Trichkova T., Ceirans A., Grabowski M. First insights into the molecular population structure and origins of the invasive Chinese sleeper, Perccottus glenii, in Europe. NeoBiota, 2020, vol. 57, pp. 87–107. https://doi.org/10.3897/neobiota.57.48958Korljakov K. A., Nohrin D. Ju. Trends in the emergence of the Volga–Ob invasive corridor. Bulletin of the Council of Young Scientists and Specialists of the Chelyabinsk Region, 2014, no. 2, pp. 19–38 (in Russian).Korljakov K. A., Nohrin D. Ju. Trends in the emergence of the Volga–Ob invasive corridor. Bulletin of the Council of Young Scientists and Specialists of the Chelyabinsk Region, 2014, no. 2, pp. 19–38 (in Russian).Kuderskij L. A. Rotan in ponds of Gorky region. In: Rybokhozjajstvennoe izuchenie vnutrennikh vodoemov [Fishery Study of Inland Water Bodies]. Leningrad, GosNIORH Publ., 1980, iss. 25, pp. 28–33 (in Russian).Kuderskij L. A. Rotan in ponds of Gorky region. In: Rybokhozjajstvennoe izuchenie vnutrennikh vodoemov [Fishery Study of Inland Water Bodies]. Leningrad, GosNIORH Publ., 1980, iss. 25, pp. 28–33 (in Russian).Luca M., Urech D., Nicuta D., Ghiorghiță G., Druică R. C., Gorgan L. D. The genetic variability of the invasive Perccottus glenii from Siret River, using the cytochrome b gene. Annals of the Romanian Society for Cell Biology, 2014, vol. 19, no. 1, pp. 11–20. https://doi.org/10.ANN/RSCB-2014-0015:RSCBLuca M., Urech D., Nicuta D., Ghiorghiță G., Druică R. C., Gorgan L. D. The genetic variability of the invasive Perccottus glenii from Siret River, using the cytochrome b gene. Annals of the Romanian Society for Cell Biology, 2014, vol. 19, no. 1, pp. 11–20. https://doi.org/10.ANN/RSCB-2014-0015:RSCBReshetnikov A. N. Influence of introduced fish Percottus glenii (Odontobutidae, Pisces). Journal of General Biology, 2001, vol. 62, no. 4, pp. 352–361.Reshetnikov A. N. Influence of introduced fish Percottus glenii (Odontobutidae, Pisces). Journal of General Biology, 2001, vol. 62, no. 4, pp. 352–361.Reshetnikov A. N. The current range of Amur sleeper Perccottus glenii Dybowski, 1877 (Odontobutidae, Pisces) in Eurasia. Russian Journal of Biological Invasions, 2010, vol. 1, iss. 2, pp. 119–126. https://doi.org/10.1134/s2075111710020116Reshetnikov A. N. The current range of Amur sleeper Perccottus glenii Dybowski, 1877 (Odontobutidae, Pisces) in Eurasia. Russian Journal of Biological Invasions, 2010, vol. 1, iss. 2, pp. 119–126. https://doi.org/10.1134/s2075111710020116Reshetnikov A. N., Chibilev E. A. Distribution of the fish rotan (Perccottus glenii Dybowski, 1877) in the Irtysh River basin and analysis of possible consequences for environment and people. Contemporary Problems of Ecology, 2009, vol. 2, no. 3, pp. 224–228. https://doi.org/10.1134/S1995425509030102Reshetnikov A. N., Chibilev E. A. Distribution of the fish rotan (Perccottus glenii Dybowski, 1877) in the Irtysh River basin and analysis of possible consequences for environment and people. Contemporary Problems of Ecology, 2009, vol. 2, no. 3, pp. 224–228. https://doi.org/10.1134/S1995425509030102Reshetnikov A. N., Ficetola G. F. Potential range of the invasive fish rotan (Perccottus glenii) in the Holarctic. Biological Invasions, 2011, vol. 13, iss. 12, pp. 2967–2980. https://doi.org/10.1007/s10530-011-9982-1Reshetnikov A. N., Ficetola G. F. Potential range of the invasive fish rotan (Perccottus glenii) in the Holarctic. Biological Invasions, 2011, vol. 13, iss. 12, pp. 2967–2980. https://doi.org/10.1007/s10530-011-9982-1Reshetnikov A. N., Petlina A. P. Distribution of the fish rotan (Perccottus glenii Dybowski, 1877) in the Ob' River. Contemporary Problems of Ecology, 2007, vol. 14, no. 4, pp. 551–556 (in Russian).Reshetnikov A. N., Petlina A. P. Distribution of the fish rotan (Perccottus glenii Dybowski, 1877) in the Ob' River. Contemporary Problems of Ecology, 2007, vol. 14, no. 4, pp. 551–556 (in Russian).Reshetnikov A. N., Sokolov S. G., Chikhlyaev I. V., Fayzulin A. I., Kirillov A. A., Kuzovenko A. E., Protasova E. N., Skomorokhov M. O. Direct and indirect interactions between an invasive alien fish (Perccottus glenii) and two native semi-aquatic snakes. Copeia, 2013, vol. 2013, pp. 103–110. https://doi.org/10.1643/CE-12-007Reshetnikov A. N., Sokolov S. G., Chikhlyaev I. V., Fayzulin A. I., Kirillov A. A., Kuzovenko A. E., Protasova E. N., Skomorokhov M. O. Direct and indirect interactions between an invasive alien fish (Perccottus glenii) and two native semi-aquatic snakes. Copeia, 2013, vol. 2013, pp. 103–110. https://doi.org/10.1643/CE-12-007Reshetnikov A. N., Golubtsov A. S., Zhuravlev V. B., Lomakin S. L., Rezvyi A. S. Range expansion of Rotan Perccottus glenii, Sunbleak Leucaspius delineatus, and Bleak Alburnus alburnus in the Ob River Basin. Contemporary Problems of Ecology, 2017, vol. 10, no. 6, pp. 612–620. https://doi.org/10.1134/S1995425517060105Reshetnikov A. N., Golubtsov A. S., Zhuravlev V. B., Lomakin S. L., Rezvyi A. S. Range expansion of Rotan Perccottus glenii, Sunbleak Leucaspius delineatus, and Bleak Alburnus alburnus in the Ob River Basin. Contemporary Problems of Ecology, 2017, vol. 10, no. 6, pp. 612–620. https://doi.org/10.1134/S1995425517060105Ruchin А. B., Osipov V. V., Fayzulin A. I., Bakin О. V., Tselishcheva L. G., Bayanov N. G. Chinese sleeper (Perccottus glenii Dybowski, 1877) (Pisces, Odontobutidae) in the reserves and national parks of the middle and lower Volga (Russia): Mini-review. AACL Bioflux, 2019, vol. 12, no. 4, pp. 1114–1124.Ruchin А. B., Osipov V. V., Fayzulin A. I., Bakin О. V., Tselishcheva L. G., Bayanov N. G. Chinese sleeper (Perccottus glenii Dybowski, 1877) (Pisces, Odontobutidae) in the reserves and national parks of the middle and lower Volga (Russia): Mini-review. AACL Bioflux, 2019, vol. 12, no. 4, pp. 1114–1124.Salmenkova E. A. Population genetic processes in introduction of fish. Russian Journal of Genetics, 2008, vol. 44, iss. 7, pp. 758–766. https://doi.org/10.1134/S1022795408070028Salmenkova E. A. Population genetic processes in introduction of fish. Russian Journal of Genetics, 2008, vol. 44, iss. 7, pp. 758–766. https://doi.org/10.1134/S1022795408070028Seleznev V. V. Low-value and trash fish species of the Chinese complex in the Kapchagay reservoir. In: Fish Resources of Water Bodies of Kazakhstan and Their Use. Alma-Ata, Kainar, 1974, iss. 8, pp. 143–148 (in Russian).Seleznev V. V. Low-value and trash fish species of the Chinese complex in the Kapchagay reservoir. In: Fish Resources of Water Bodies of Kazakhstan and Their Use. Alma-Ata, Kainar, 1974, iss. 8, pp. 143–148 (in Russian).Semenov D. Ju. Antropogennaya transformatsiya ihtiofauny Sredney Volgi v Kujbyshevskom vodohranilishche [Anthropogenic Transformation of the Ichthyofauna of the Middle Volga in the Kuibyshev Reservoir]. Ulyanovsk, Ulyanovsk State University Publ., 2011. 114 p. (in Russian).Semenov D. Ju. Antropogennaya transformatsiya ihtiofauny Sredney Volgi v Kujbyshevskom vodohranilishche [Anthropogenic Transformation of the Ichthyofauna of the Middle Volga in the Kuibyshev Reservoir]. Ulyanovsk, Ulyanovsk State University Publ., 2011. 114 p. (in Russian).Shamov A. G. Goloveshka-rotan in the Kuibyshev reservoir. In: Problemy okhrany vod i rybnykh resursov: tezisy dokladov III Povolzhskoi konferentsii [Problems of Protection of Water and Fish Resources: Theses of reports of the III Volga Conference]. Kazan, Kazan State Pedagogical Institute Publ., 1983, pp. 147–148 (in Russian).Shamov A. G. Goloveshka-rotan in the Kuibyshev reservoir. In: Problemy okhrany vod i rybnykh resursov: tezisy dokladov III Povolzhskoi konferentsii [Problems of Protection of Water and Fish Resources: Theses of reports of the III Volga Conference]. Kazan, Kazan State Pedagogical Institute Publ., 1983, pp. 147–148 (in Russian).Shashulovskii V. A., Ermolin V. P. Ichthyofauna composition of the Volgograd Reservoir. Voprosy ikhtiologii, 2005, vol. 45, no. 3, pp. 324–330 (in Russian).Shashulovskii V. A., Ermolin V. P. Ichthyofauna composition of the Volgograd Reservoir. Voprosy ikhtiologii, 2005, vol. 45, no. 3, pp. 324–330 (in Russian).Spanovskaya V. D., Savvaitova K. A., Potapova T. L. About variability of rotan (Perccottus glehni Dyb fam. Eleotridae) under acclimatization. Voprosy ikhtiologii, 1964, vol. 4, no. 4, pp. 632–643 (in Russian).Spanovskaya V. D., Savvaitova K. A., Potapova T. L. About variability of rotan (Perccottus glehni Dyb fam. Eleotridae) under acclimatization. Voprosy ikhtiologii, 1964, vol. 4, no. 4, pp. 632–643 (in Russian).Susliaev V. V., Reshetnikova S. N., Interesova E. A. Biology of rotan gobi Perccottus glenii Dybowski, 1877 in water basins of south-taiga zone of Western Siberia. Bulletin of Novosibirsk State Agrarian University, 2016, vol. 1, pp. 78–85 (in Russian).Susliaev V. V., Reshetnikova S. N., Interesova E. A. Biology of rotan gobi Perccottus glenii Dybowski, 1877 in water basins of south-taiga zone of Western Siberia. Bulletin of Novosibirsk State Agrarian University, 2016, vol. 1, pp. 78–85 (in Russian).Xu W., Yin W., Chen A., Li J., Lei G., Fu C. Phylogeographical analysis of a cold-temperate freshwater fish, the Amur sleeper (Perccottus glenii) in the Amur and Liaohe River basins of Northeast Asia. Zoological Science, 2014, vol. 31, no. 10, pp. 671–679. https://doi.org/10.2108/zs130046Xu W., Yin W., Chen A., Li J., Lei G., Fu C. Phylogeographical analysis of a cold-temperate freshwater fish, the Amur sleeper (Perccottus glenii) in the Amur and Liaohe River basins of Northeast Asia. Zoological Science, 2014, vol. 31, no. 10, pp. 671–679. https://doi.org/10.2108/zs130046Yadrenkina E. N. Distribution of alien fish species in lakes within the temperate climatic zone of Western Siberia. Russian Journal of Biological Invasions, 2012, vol. 3, iss. 2, pp. 145– 157. https://doi.org/10.1134/S2075111712020117Yadrenkina E. N. Distribution of alien fish species in lakes within the temperate climatic zone of Western Siberia. Russian Journal of Biological Invasions, 2012, vol. 3, iss. 2, pp. 145– 157. https://doi.org/10.1134/S2075111712020117Yang P., Hu Z., Liu Y., Jin G., Wang L. Mitochondrial genetic diversity and population structure of the Amur sleeper (Perccottus glenii) in Northeast China. Pakistan Journal of Zoology, 2020, vol. 52, iss. 4, pp. 1531–1539. https://doi.org/10.17582/journal.pjz/20190528220510Yang P., Hu Z., Liu Y., Jin G., Wang L. Mitochondrial genetic diversity and population structure of the Amur sleeper (Perccottus glenii) in Northeast China. Pakistan Journal of Zoology, 2020, vol. 52, iss. 4, pp. 1531–1539. https://doi.org/10.17582/journal.pjz/20190528220510Yeh F. C., Yang R., Boyle T. Popgene. Version 1.31. Edmonton, University of Alberta and Centre for International Forestry Research. 1999. Available at: http://www.ualberta.ca/~fyeh/ popgene_download.html (accessed July 25, 2024).Yeh F. C., Yang R., Boyle T. Popgene. Version 1.31. Edmonton, University of Alberta and Centre for International Forestry Research. 1999. Available at: http://www.ualberta.ca/~fyeh/ popgene_download.html (accessed July 25, 2024).Zhang Y., Sun J., Shi L., Yu H., Ji Z. Population genetic pattern of the freshwater fish Amur sleeper (Perccottus glenii) across its native distribution area in China. Conservation Genetics, 2021, vol. 22, iss. 1, pp. 125–131. https://doi.org/10.1007/s10592-020-01323-0Zhang Y., Sun J., Shi L., Yu H., Ji Z. Population genetic pattern of the freshwater fish Amur sleeper (Perccottus glenii) across its native distribution area in China. Conservation Genetics, 2021, vol. 22, iss. 1, pp. 125–131. https://doi.org/10.1007/s10592-020-01323-0Zhigileva O. N., Alyamkin G. V. Genetic polymorphism of invasive populations of Amur sleeper Perccottus glenii (Perciformes: Odontobutidae) from the Irtysh basin determined by the sequencing of the Cytb gene region. Russian Journal of Biological Invasions, 2024, vol. 15, iss. 3, pp. 318–323. https://doi.org/10.1134/S2075111724700206Zhigileva O. N., Alyamkin G. V. Genetic polymorphism of invasive populations of Amur sleeper Perccottus glenii (Perciformes: Odontobutidae) from the Irtysh basin determined by the sequencing of the Cytb gene region. Russian Journal of Biological Invasions, 2024, vol. 15, iss. 3, pp. 318–323. https://doi.org/10.1134/S2075111724700206Zhigileva O. N., Kulikova A. A. Specific biological features and genetic variation of Chinese sleeper Perccottus glenii (Odontobutidae) in aquatic bodies of Tyumen oblast. Journal of Ichthyology, 2016, vol. 56, no. 1, pp. 124–132. https://doi.org/10.1134/S0032945216010173Zhigileva O. N., Kulikova A. A. Specific biological features and genetic variation of Chinese sleeper Perccottus glenii (Odontobutidae) in aquatic bodies of Tyumen oblast. Journal of Ichthyology, 2016, vol. 56, no. 1, pp. 124–132. https://doi.org/10.1134/S0032945216010173Zietjiewicz E., Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994, vol. 20, no. 2, pp. 176–183.Zietjiewicz E., Rafalski A., Labuda D. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 1994, vol. 20, no. 2, pp. 176–183.

The authors declare that there are no conflicts of interest present.