sevinПОВОЛЖСКИЙ ЭКОЛОГИЧЕСКИЙ ЖУРНАЛPovolzhskiy Journal of Ecology1684-73182541-8963Федеральное государственное бюджетное учреждение науки Институт проблем экологии и эволюции им. А. Н. Северцова РАН; Товарищество научных изданий КМК.10.35885/1684-7318-2022-3-322-335sevin-370Research ArticleСТАТЬИARTICLESНовые энергетические подходы использования отработанных биосорбентов микроводорослей Chlorella kessleri (Chlorellaceae, Chlorellales)New energy approaches to the use of waste biosorbents of microalgae Chlorella kessleri (Chlorellaceae, Chlorellales)https://orcid.org/0000-0002-5914-6210ПолитаеваН. А.PolitaevaN. A.

Политаева Наталья Анатольевна

195251, г. Санкт-Петербург, ул. Политехническая, д. 29

Natalia A. Politaeva

Polytechnicheskaya St., Saint Petersburg 195251

politaevana1971@gmail.comhttps://orcid.org/0000-0002-1834-4894ИльинИ. В.IllinI. V.

Ильин Игорь Васильевич

195251, г. Санкт-Петербург, ул. Политехническая, д. 29

Igor V. Illin

29 Polytechnicheskaya St., Saint Petersburg 195251

igor.ilin@spbstu.ruhttps://orcid.org/0000-0002-2043-1866ОпаринаА. М.OparinaA. M.

Опарина Анна Михайловна

195251, г. Санкт-Петербург, ул. Политехническая, д. 29

Anna M. Oparina

29 Polytechnicheskaya St., Saint Petersburg 195251

annaoparina93@gmail.comhttps://orcid.org/0000-0002-3702-9945ДонецковаА. С.DonetskovaA. S.

Донецкова Анастасия Сергеевна

195251, г. Санкт-Петербург, ул. Политехническая, д. 29

Anastasia S. Donetskova

29 Polytechnicheskaya St., Saint Petersburg 195251

donetsckova.anastasia@yandex.ruСанкт-Петербургский политехнический университет Петра ВеликогоРоссияPeter the Great St. Petersburg Polytechnic UniversityRussian Federation20221311202203322335Copyright © Политаева Н.А., Ильин И.В., Опарина А.М., Донецкова А.С., 20222022Политаева Н.А., Ильин И.В., Опарина А.М., Донецкова А.С.Politaeva N.A., Illin I.V., Oparina A.M., Donetskova A.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://sevin.elpub.ru/jour/article/view/370

Рассматривается использование микроводорослей Chlorella kessleri ВКПМ А1-11 ARM (РФ, НПО «Алгобиотехнология») для экологических и энергетических целей. Приводятся результаты исследования применения биомассы микроводоросли C. kessleri в качестве биосорбента для очистки модельных сточных вод от ионов Cu2+ в статических условиях. Биосорбция – перспективная технология очистки промышленных стоков, содержащих в себе различные соединения тяжелых металлов, однако вопросы экономической выгоды использования биосорбентов, их экологическая безопасность и стоимость утилизации отработанных сорбентов подвергаются большому обсуждению. Предлагается утилизировать отработанный биосорбент, образующийся после очистки сточных вод от меди как дополнительное топливо. Концентрация меди в фильтрате определена методом колориметрического анализа с диэтилдитиокарбаматом натрия. Эффективность очистки и сорбционная емкость сухой массы C. kessleri получена расчетным путем. Максимальная сорбционная емкость к ионам Cu2+ составила 4.2 мг/г. Эффективность очистки достигала 87% при начальной концентрации ионов Cu2+ = 97 мг/л. Испытания по определению удельной теплоты сгорания биомассы C. kessleri и отработанных биосорбентов на ее основе проведены калориметрическим методом при помощи калориметра бомбы. Удельная теплота сгорания равна 22125 и 21674 кДж/кг соответственно. Приведено сравнение данных величин с традиционными энергоносителями. Разработана технологическая схема безотходного цикла использования C. kessleri для очистки сточных вод промышленных предприятий с получением в качестве конечных продуктов нескольких ценных ресурсов, таких как очищенная вода, энергетические р

The use of microalgae Chlorella kessleri VKPM A1-11 ARM (RF, NPO Algobiotechnology) for environmental and energy purposes is considered. The results of our study of the use of C. kessleri microalgae biomass as a biosorbent to purify model wastewater from Cu2+ ions under static conditions are presented. Biosorption is a promising technology for the treatment of industrial effluents containing various heavy metal compounds, but the issues of economic benefits of using biosorbents, their environmental safety and the cost of disposal of used sorbents are subject to much discussion. The paper proposes to dispose the used biosorbent formed after wastewater treatment from copper as an additional fuel. The copper concentration in the filtrate was determined by colorimetric analysis with sodium diethyldithiocarbamate. The cleaning efficiency and sorption capacity of the dry mass of C. kessleri were obtained by calculation. The maximum sorption capacity for Cu2+ ions was 4.2 mg/g. The purification efficiency reached 87% at the initial concentration of Cu2+ ions being 97 mg/l. Tests to estimate the specific heat of combustion of C. kessleri biomass and used biosorbents based thereon were carried out by the calorimetric method using a bomb calorimeter. The specific heats of combustion were 22,125 kJ/kg and 21,674 kJ/kg, respectively. A comparison of these values with traditional energy carriers is given. A technological scheme has been developed for a waste-free cycle of using C. kessleri to treat wastewater from industrial enterprises with the production of several valuable resources as end products, such as purified water, energy resources, fertilizers, and recycled metals. The obtained results of our study can be applied in technologies for post-treatment of wastewater from various industrial enterprises using biological non-waste resources.

микроводоросль Chlorellaтяжелые металлысточные водыбиосорбциябезотходное использованиеmicroalgae Chlorellaheavy metalswastewaterbiosorptionwaste-free useРабота выполнена при поддержке Министерства науки и высшего образования Российской Федерации в рамках проекта «Технологические вызовы и социальноэкономические преобразования в контексте зеленых переходов» (Соглашение № 75-15-2022-1136 от 01.07.2022).The research was supported by the Ministry of Science and Higher Education of the Russian Federation under the project “Technological Challenges and Socioeconomic Transformation in the Context of Green Transitions” (Agreement No. 75-15-2022-1136 dated 01.07.2022).ReferencesГОСТ 4388-72. Вода питьевая. Методы определения массовой концентрации меди. М. : Изд-во стандартов, 1986. 12 с.GOST 4388-72. Drinking water. Methods for determination of copper content. Moscow, Izdatel'stvo standartov, 1986. 12 p. (in Russian).Зибарев Н. В., Политаева Н. А., Андрианова М. Ю. Использование микроводорослей Chlorella sorokiniana (Chlorellaceae, Chlorellales) для очистки сточных вод пивоваренной промышленности // Поволжский экологический журнал. 2021. № 3. С. 262 – 271. https://doi.org/10.35885/1684-7318-2021-3-262-271Zibarev N. V., Politaeva N. A., Andrianova M. Yu. Use of Chlorella sorokiniana (Chlorellaceae, Chlorellales) microalgae for purification of waste water from the brewing industry. Povolzhskiy Journal of Ecology, 2021, no. 3, pp. 262–271 (in Russian). https://doi.org/10.35885/1684-7318-2021-3-262-271Ольшанская Л. Н., Собгайда Н. А., Валиев Р. Ш. Извлечение тяжелых металлов из загрязненных стоков с использованием адсорбентов и фитосорбентов // Экология и промышленность России. 2015. Т. 19, № 11. С. 18 – 23. https://doi.org/10.18412/1816-0395-2015-11-18-23Ol'shanskaya L. N., Sobgaуda N. A., Valiуev R. Sh. Extraction of heavy metals from polluted waters with adsorbents and phytosorbents. Ecology and Industry of Russia, 2015, vol. 19, no. 11, pp. 18–23 (in Russian). https://doi.org/10.18412/1816-0395-2015-11-18-23Смятская Ю. А., Фазуллина А. А., Политаева Н. А., Жажков В. В., Павлушкина Ю. Е.,Smyatskaya Yu. A., Fazullina A. A., Politaeva N. A., Zhazhkov V. V., Pavlushkina Yu. E., Dolbnya I. V. The use and utilization of chitosan sorbents – The residual biomass of microalgae Chlorella sorokiniana. Ecology and Industry of Russia, 2019 a, vol. 23, no. 9, pp. 18–23 (in Russian). https://doi.org/10.18412/1816-0395-2019-9-18-23Долбня И. В. Использование и утилизация сорбентов хитозан – остаточная биомасса микроводорослей Chlorella sorokiniana // Экология и промышленность России. 2019 а. Т. 23, № 9. С. 18 – 23. https://doi.org/10.18412/1816-0395-2019-9-18-23Smyatskaya Yu. A., Fazullina A. A., Politaeva N. A., Chusov A. N., Bezborodov A. A. Wastewater treatment of iron(III) Ions with residual biomass of microalgae Сhlorella sorokiniana. Ecology and Industry of Russia, 2019 b, vol. 23, no. 6, pp. 22–27 (in Russian). https://doi.org/10.18412/1816-0395-2019-6-22-27Смятская Ю. А., Фазуллина А. А., Политаева Н. А., Чусов А. Н., Безбородов А. А. Очистка сточных вод от ионов железа(III) остаточной биомассой микроводорослей Chlorella sorokiniana // Экология и промышленность России. 2019 б. Т. 23, № 6. С. 22 – 27. https://doi.org/10.18412/1816-0395-2019-6-22-27 Физическая энциклопедия: в 5 т. / гл. ред. А. М. Прохоров. М. : Советская энциклопедия, 1988 – 1990. Т. 1 – 2; Большая Российская энциклопедия, 1992 – 1998. Т. 3 – 5.Fizicheskaia entsiklopediia: v 5 t. Gl. red. A. M. Prokhorov [Prokhorov A. M., ed. The Physical Encyclopedia: in 5 volumes]. Moscow, Sovetskaia entsiklopediia Publ., 1988–1990, vol. 1–2; Bol'shaia Rossiiskaia entsiklopediia Publ., 1992–1998, vol. 3–5 (in Russian).Abu Al-Rub F. A., El-Naas M. H., Ashour I., Al-Marzouqi M. Biosorption of copper on Chlorella vulgaris from single, binary and ternary metal aqueous solutions // Process Biochemistry. 2006. Vol. 41, iss. 2. P. 457 – 464. https://doi.org/10.1016/j.procbio.2005.07.018Abu Al-Rub F. A., El-Naas M. H., Ashour I., Al-Marzouqi M. Biosorption of copper on Chlorella vulgaris from single, binary and ternary metal aqueous solutions. Process Biochemistry, 2006, vol. 41, iss. 2, pp. 457–464. https://doi.org/10.1016/j.procbio.2005.07.018Banfalvi G. Cellular Effects of Heavy Metals. Dordrecht : Springer, 2011. 348 p. https://doi.org/10.1007/978-94-007-0428-2Banfalvi G. Cellular Effects of Heavy Metals. Dordrecht, Springer, 2011. 348 p. https://doi.org/10.1007/978-94-007-0428-2Danouche M., El Ghachtouli N., El Arroussi H. Phycoremediation mechanisms of heavy metals using living green microalgae: Physicochemical and molecular approaches for enhancing selectivity and removal capacity // Heliyon. 2021. Vol. 7, iss. 7. Article number e07609. https://doi.org/10.1016/j.heliyon.2021.e07609Danouche M., El Ghachtouli N., El Arroussi H. Phycoremediation mechanisms of heavy metals using living green microalgae: Physicochemical and molecular approaches for enhancing selectivity and removal capacity. Heliyon, 2021, vol. 7, iss. 7, article number e07609. https://doi.org/10.1016/j.heliyon.2021.e07609Gupta S. K., Ansari F. A., Shriwastav A., Sahoo N. K., Rawat I., Bux F. Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for biofuels // Journal of Cleaner Production. 2016. Vol. 115. P. 255 – 264. https://doi.org/10.1016/j.jclepro.2015.12.040Gupta S. K., Ansari F. A., Shriwastav A., Sahoo N. K., Rawat I., Bux F. Dual role of Chlorella sorokiniana and Scenedesmus obliquus for comprehensive wastewater treatment and biomass production for biofuels. Journal of Cleaner Production, 2016, vol. 115, pp. 255–264. https://doi.org/10.1016/j.jclepro.2015.12.040Gupta V. K., Ali I., Saleh T. A., Nayak A., Agarwal S. Chemical treatment technologies for waste-water recyclingan overview // RSC Advances. 2012. Vol. 16. P. 6380 – 6388. https://doi.org/10.1039/c2ra20340eGupta V. K., Ali I., Saleh T. A., Nayak A., Agarwal S. Chemical treatment technologies for waste-water recyclingan overview. RSC Advances, 2012, vol. 16, pp. 6380–6388. https://doi.org/10.1039/c2ra20340eIslam S. M. D., Huda M. E. Water Pollution by industrial effluent and phytoplankton diversity of Shitalakhya River, Bangladesh // Journal of Scientific Research. 2016. Vol. 8, no. 2. P. 191 – 198. https://doi.org/10.3329/jsr.v8i2.26402Islam S. M. D., Huda M. E. Water Pollution by industrial effluent and phytoplankton diversity of Shitalakhya River, Bangladesh. Journal of Scientific Research, 2016, vol. 8, no. 2, pp. 191–198. https://doi.org/10.3329/jsr.v8i2.26402Javanbakht V., Alavi S. A., Zilouei H. Mechanisms of heavy metal removal using microorganisms as biosorbent // Water Science & Technology. 2014. Vol. 69, no. 9. P. 1775 – 1787. https://doi.org/ 10.2166/wst.2013.718Javanbakht V., Alavi S. A., Zilouei H. Mechanisms of heavy metal removal using microorganisms as biosorbent. Water Science & Technology, 2014, vol. 69, no. 9, pp. 1775–1787. https://doi.org/10.2166/wst.2013.718Li R., Wen Y., Lin G., Meng C., He P., Wang F. Different sources of copper effect on intestinal epithelial cell: Toxicity, oxidative stress, and metabolism // Metabolites. 2019. Vol. 10, no. 1. Article number 11. https://doi.org/10.3390/metabo10010011Li R., Wen Y., Lin G., Meng C., He P., Wang F. Different sources of copper effect on intestinal epithelial cell: Toxicity, oxidative stress, and metabolism. Metabolites, 2019, vol. 10, no. 1, article number 11. https://doi.org/10.3390/metabo10010011Makareviciene V., Sendzikiene E. Application of microalgae biomass for biodiesel fuel production // Energies. 2022. Vol. 15, no. 11. P. 1 – 33. https://doi.org/10.3390/en15114178Makareviciene V., Sendzikiene E. Application of microalgae biomass for biodiesel fuel production. Energies, 2022, vol. 15, no. 11, pp. 1–33. https://doi.org/10.3390/en15114178Malhotra N., Ger T. R., Uapipatanakul B., Huang J. C., Chen K. H., Hsiao C. D. Review of copper and copper nanoparticle toxicity in fish // Nanomaterials. 2020. Vol. 10, iss. 6. Article number 1126. https://doi.org/10.3390/nano10061126Malhotra N., Ger T. R., Uapipatanakul B., Huang J. C., Chen K. H., Hsiao C. D. Review of copper and copper nanoparticle toxicity in fish. Nanomaterials, 2020, vol. 10, iss. 6, article number 1126. https://doi.org/10.3390/nano10061126Milinki E., Molnár S., Kiss A., Virág D., Pénzes-Kónya E. Study of microelement accumulating characteristics of microalgae // Acta Botanica Hungarica. 2011. Vol. 53, iss. 1-2. P. 159 – 167. https://doi.org/10.1556/ABot.53.2011.1-2.15Milinki E., Molnár S., Kiss A., Virág D., Pénzes-Kónya E. Study of microelement accumulating characteristics of microalgae. Acta Botanica Hungarica, 2011, vol. 53, iss. 1-2, pp. 159–167. https://doi.org/10.1556/ABot.53.2011.1-2.15Papirio S., Frunzo L., Mattei M. R., Ferraro A., Race M., D’Acunto B., Pirozzi F., Esposito G. Heavy metal removal from wastewaters by biosorption: Mechanisms and modeling // Sustainable Heavy Metal Remediation. Environmental Chemistry for a Sustainable World. Cham : Springer, 2017. Vol. 8. P. 25 – 63. https://doi.org/10.1007/978-3-319-58622-9_2Papirio S., Frunzo L., Mattei M. R., Ferraro A., Race M., D’Acunto B., Pirozzi F., Esposito G. Heavy metal removal from wastewaters by biosorption: Mechanisms and modeling. Sustainable Heavy Metal Remediation. Environmental Chemistry for a Sustainable World. Cham, Springer, 2017, vol. 8, pp. 25–63. https://doi.org/10.1007/978-3-319-58622-9_2Pattnaik P., Dangayach G. S., Bhardwaj A. K. A review on the sustainability of textile industries wastewater with and without treatment methodologies // Reviews on Environmental Health. 2018. Vol. 33, iss. 2. P. 163 – 203. https://doi.org/10.1515/reveh-2018-0013Pattnaik P., Dangayach G. S., Bhardwaj A. K. A review on the sustainability of textile industries wastewater with and without treatment methodologies. Reviews on Environmental Health, 2018, vol. 33, iss. 2, pp. 163–203. https://doi.org/10.1515/reveh-2018-0013Petrovič A., Simonič M. Removal of heavy metal ions from drinking water by alginateimmobilised Chlorella sorokiniana // International Journal of Environmental Science and Technology. 2016. Vol. 13, iss. 7. P. 1761 – 1780. https://doi.org/10.1007/s13762-016-1015-2Petrovič A., Simonič M. Removal of heavy metal ions from drinking water by alginateimmobilised Chlorella sorokiniana. International Journal of Environmental Science and Technology, 2016, vol. 13, iss. 7, pp. 1761–1780. https://doi.org/10.1007/s13762-016-1015-2Politaeva N., Kuznetsova T., Smyatskaya Y., Atamaniuk I., Trukhina E. Chlorella microalga biomass cultivation for obtaining energy in climatic conditions of St. Petersburg // International Scientific Conference Energy Management of Municipal Transportation Facilities and Transport EMMFT 2017 : Conference Proceedings. Series : Advances in Intelligent Systems and Computing. Cham : Springer, 2018. Vol. 692. P. 555 – 562. https://doi.org/10.1007/978-3-319-70987-1_59Politaeva N., Kuznetsova T., Smyatskaya Y., Atamaniuk I., Trukhina E. Chlorella microalga biomass cultivation for obtaining energy in climatic conditions of St. Petersburg. International Scientific Conference Energy Management of Municipal Transportation Facilities and Transport EMMFT 2017: Conference Proceedings. Series: Advances in Intelligent Systems and Computing. Cham, Springer, 2018, vol. 692, pp. 555–562. https://doi.org/10.1007/978-3-319-70987-1_59Politayeva N. A., Smyatskaya Y. A., Dolbnya I. V., Kasobov L. S., Rakhimov D. B., Zaripova D. A. Research of pH influence on sorption properties of sorbents on a basis of residual biomass of microalgae Chlorella sorokiniana and duckweed Lemna minor // E3S Web of Conferences : International Scientific and Technical Conference Smart Energy Systems 2019 (SES-2019). 2019. Vol. 124. Article number 01050. https://doi.org/10.1051/e3sconf/201912401050Politayeva N. A., Smyatskaya Y. A., Dolbnya I. V., Kasobov L. S., Rakhimov D. B., Zaripova D. A. Research of pH influence on sorption properties of sorbents on a basis of residual biomass of microalgae Chlorella sorokiniana and duckweed Lemna minor. E3S Web of Conferences: International Scientific and Technical Conference Smart Energy Systems 2019 (SES-2019), 2019, vol. 124, article number 01050. https://doi.org/10.1051/e3sconf/201912401050Politaeva N. A., Smyatskaya Y. A., Tatarintseva E. A. Using adsorption material based on the residual biomass of Chlorella sorokiniana microalgae for wastewater purification to remove heavy metal ions // Chemical and Petroleum Engineering. 2020. Vol. 55, no. 11-12. P. 907 – 912. https://doi.org/10.1007/s10556-020-00712-zPolitaeva N. A., Smyatskaya Y. A., Tatarintseva E. A. Using adsorption material based on the residual biomass of Chlorella sorokiniana microalgae for wastewater purification to remove heavy metal ions. Chemical and Petroleum Engineering, 2020, vol. 55, no. 11-12, pp. 907–912. https://doi.org/10.1007/s10556-020-00712-zPolitaeva N., Badenko V. Magnetic and electric field accelerate Phytoextraction of copper Lemna minor duckweed // PLoS ONE. 2021. Vol. 16, no. 8. Article number e0255512. https://doi.org/10.1371/journal.pone.0255512Politaeva N., Badenko V. Magnetic and electric field accelerate Phytoextraction of copper Lemna minor duckweed. PLoS ONE, 2021, vol. 16, no. 8, article number e0255512. https://doi.org/10.1371/journal.pone.0255512Rissoni Toledo A. G., Reyes Andrade J. C., Palmieri M. C., Bevilaqua D., Pombeiro Sponchiado S. R. Innovative method for encapsulating highly pigmented biomass from Aspergillus nidulans mutant for copper ions removal and recovery // PLoS ONE. 2021. Vol. 16, no. 11. Article number e0259315. https://doi.org/10.1371/journal.pone.0259315Rissoni Toledo A. G., Reyes Andrade J. C., Palmieri M. C., Bevilaqua D., Pombeiro Sponchiado S. R. Innovative method for encapsulating highly pigmented biomass from Aspergillus nidulans mutant for copper ions removal and recovery. PLoS ONE, 2021, vol. 16, no. 11, article number e0259315. https://doi.org/10.1371/journal.pone.0259315Sarwer A., Hamed S. M., Osman A. I., Jamil F., Al-Muhtaseb A. H., Alhajeri N. S., Rooney D. W. Algal biomass valorization for biofuel production and carbon sequestration: A review // Environmental Chemistry Letters. 2022. Vol. 20, iss. 5. P. 2797 – 2851. https://doi.org/10.1007/s10311-022-01458-1Sarwer A., Hamed S. M., Osman A. I., Jamil F., Al-Muhtaseb A. H., Alhajeri N. S., Rooney D. W. Algal biomass valorization for biofuel production and carbon sequestration: A review. Environmental Chemistry Letters, 2022, vol. 20, iss. 5, pp. 2797–2851. https://doi.org/10.1007/s10311-022-01458-1Tawfik A., Ismail S., Elsayed M., Qyyum M. A., Rehan M. Sustainable microalgal biomass valorization to bioenergy: Key challenges and future perspectives // Chemosphere. 2022. Vol. 296. Article number 133812. https://doi.org/10.1016/j.chemosphere.2022.133812Tawfik A., Ismail S., Elsayed M., Qyyum M. A., Rehan M. Sustainable microalgal biomass valorization to bioenergy: Key challenges and future perspectives. Chemosphere, 2022, vol. 296, article number 133812. https://doi.org/10.1016/j.chemosphere.2022.133812Vardhan K. H., Kumar P. S., Panda R. C. A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives // Journal of Molecular Liquids. 2019. Vol. 290. Article number 111197. https://doi.org/10.1016/j.molliq.2019.111197Vardhan K. H., Kumar P. S., Panda R. C. A review on heavy metal pollution, toxicity and remedial measures: Current trends and future perspectives. Journal of Molecular Liquids, 2019, vol. 290, article number 111197. https://doi.org/10.1016/j.molliq.2019.111197Worch E. Adsorption Technology in Water Treatment : Fundamentals, Processes, and Modeling. Berlin ; Boston : De Gruyter, 2012. 332 p. https://doi.org/10.1515/9783110240238Worch E. Adsorption Technology in Water Treatment: Fundamentals, Processes, and Modeling. Berlin, Boston, De Gruyter, 2012. 332 p. https://doi.org/10.1515/9783110240238

The authors declare that there are no conflicts of interest present.