<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">sevin</journal-id><journal-title-group><journal-title xml:lang="ru">ПОВОЛЖСКИЙ ЭКОЛОГИЧЕСКИЙ ЖУРНАЛ</journal-title><trans-title-group xml:lang="en"><trans-title>Povolzhskiy Journal of Ecology</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">1684-7318</issn><issn pub-type="epub">2541-8963</issn><publisher><publisher-name>Федеральное государственное бюджетное учреждение науки Институт проблем экологии и эволюции им. А. Н. Северцова РАН; Товарищество научных изданий КМК.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.35885/1684-7318-2021-3-262-271</article-id><article-id custom-type="elpub" pub-id-type="custom">sevin-276</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Использование микроводорослей Chlorella sorokiniana (Chlorellaceae, Chlorellales) для очистки сточных вод пивоваренной промышленности</article-title><trans-title-group xml:lang="en"><trans-title>Use of Chlorella sorokiniana (Chlorellaceae, Chlorellales) microalgae for purification of waste water from the brewing industry</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8710-6304</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зибарев</surname><given-names>Н. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Zibarev</surname><given-names>N. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Зибарев Никита Васильевич</p><p>195251, г. Санкт-Петербург, ул. Политехническая, д. 29</p></bio><bio xml:lang="en"><p>Nikita V. Zibarev</p><p>29 Polytechnicheskaya St., Saint Petersburg 195251</p></bio><email xlink:type="simple">ij1995@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-5914-6210</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Политаева</surname><given-names>Н. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Politaeva</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Политаева Наталья Анатольевна</p><p>195251, г. Санкт-Петербург, ул. Политехническая, д. 29</p></bio><bio xml:lang="en"><p>Natalia A. Politaeva</p><p>29 Polytechnicheskaya St., Saint Petersburg 195251</p></bio><email xlink:type="simple">politaevana1971@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5723-851X</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Андрианова</surname><given-names>М. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Andrianova</surname><given-names>M. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>195251, г. Санкт-Петербург, ул. Политехническая, д. 29</p></bio><bio xml:lang="en"><p>Maria Yu. Andrianova</p><p>29 Polytechnicheskaya St., Saint Petersburg 195251</p></bio><email xlink:type="simple">andrianova_myu@spbstu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Санкт-Петербургский политехнический университет Петра Великого</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Peter the Great St. Petersburg Polytechnic University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2021</year></pub-date><pub-date pub-type="epub"><day>23</day><month>11</month><year>2021</year></pub-date><volume>0</volume><issue>3</issue><fpage>262</fpage><lpage>271</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Зибарев Н.В., Политаева Н.А., Андрианова М.Ю., 2021</copyright-statement><copyright-year>2021</copyright-year><copyright-holder xml:lang="ru">Зибарев Н.В., Политаева Н.А., Андрианова М.Ю.</copyright-holder><copyright-holder xml:lang="en">Zibarev N.V., Politaeva N.A., Andrianova M.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://sevin.elpub.ru/jour/article/view/276">https://sevin.elpub.ru/jour/article/view/276</self-uri><abstract><p>Представлены экологические проблемы пивоваренного производства. Изучены литературные данные по способам очистки вод с применением микроводорослей. Показано, что состав сточных вод в пивоваренной промышленности состоит из биогенных элементов азота, фосфора и калия, которые необходимы для культивирования биомассы микроводорослей, поэтому сточные воды возможно использовать как основу для создания питательной среды культивирования микроводорослей. В экспериментальной части изучали влияние различного разбавления суспензии микроводорослей Chlorella sorokiniana (Х), выращенных на питательной среде с неочищенными сточными водами пивоваренной промышленности (СВ). Изучали различные соотношения СВ к суспензии микроводорослей в процентном соотношении 70:30, 50:50, 30:70 на способность поглощать из СВ азот, органический и неорганический углерод, изменять величину рН. Представлены зависимости скорости роста микроводорослей при различном процентном соотношении добавки СВ пивоваренной промышленности и питательной среды. Показано, что при соотношении 30:70 эта зависимость характерна для стандартной кривой роста культуры микроорганизмов. Прослеживаются лаг-фаза, фазы экспоненциального роста и отмирания. При отношении 70:30 очевидно пагубное влияние стоков на рост микроводорослей: на третьи сутки происходит их гибель, зеленый раствор приобретает коричневую окраску, что подтверждает гибель клеток микроводорослей. Показано, что оптимальным соотношением является 30:70, при данном соотношении не происходит гибели биомассы, она хорошо растет, используя загрязняющие вещества для своего питания. Цвет раствора после культивирования имел ярко-зеленую окраску, что соответствует цвету здоровых клеток. При соотношении 30:70 происходит очистка сточных вод по общему азоту до 70%, для органического углерода – до 90%. Величина рН сменяется с кислого значения до нейтрального. </p></abstract><trans-abstract xml:lang="en"><p>The paper presents some environmental problems of the brewing industry. The literature data on the methods of water purification using microalgae have been studied. The composition of wastewater from the brewing industry is shown to consist of few biogenic elements, namely: nitrogen, phosphorus and potassium, which are necessary for microalgae biomass cultivation. Therefore, the wastewater from the brewing industry can be used as a basis for making a nutrient medium to cultivate microalgae. In the experimental part, the effect of various dilutions of a suspension of microalgae Chlorella sorokiniana (C) grown on a nutrient medium with untreated wastewater (UWW) from the brewing industry was studied. Various percentage ratios of wastewater to microalga suspension were studied, namely: UWW/C = 70:30, 50:50, and 30:70 for the ability to absorb nitrogen, organic and inorganic carbon from dry matter, and to change the pH value. The dependences of the growth rate of microalgae at various percentage additives of the wastewater from the brewing industry are presented. It was shown that at a UWW:C = 30:70, this dependence is characteristic of the standard growth curve of a microorganism culture. A lag phase, phases of exponential growth and withering away are observed. At a 70:30 ratio, the harmful effect of effluents on the growth of microalgae is obvious, on the 3rd day their death occurs, the green solution acquiring a brown color to confirm the death of microalgae cells. It has been shown that the optimal UWW:C ratio is 30:70, with no loss of biomass, it grows well using pollutants for its nutrition. The color of the solution after cultivation was bright green, which corresponded to the color of healthy cells. At UWW:C = 30:70, the wastewater is purified up to 70% and 90% for total nitrogen and organic carbon, respectively. The pH value changes from acidic to neutral. Keywords: Chlorella sorokiniana, wastewater treatment, brewing industry</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Chlorella sorokiniana</kwd><kwd>очистка сточных вод</kwd><kwd>пивоваренная промышленность</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Chlorella sorokiniana</kwd><kwd>wastewater treatment</kwd><kwd>brewing industry</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Мамедова Т. Т. Различные подходы к накоплению биомассы водорослей Chlorella vulgaris и процессам ее биокаталитической трансформации : дис. … канд. хим. наук. М., 2015. 176 с.</mixed-citation><mixed-citation xml:lang="en">Mammadova T. T. Razlichnye podkhody k nakopleniiu biomassy vodoroslei Chlorella vulgaris i protsessam ee biokataliticheskoi transformatsii [Various Approaches to the Accumulation of Algae Biomass of Chlorella vulgaris and the Processes of its Biocatalytic Transformation]. Diss. Cand. Sci. (Biol.). Moscow, 2015. 176 p. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Политаева Н. А., Смятская Ю. А., Кузнецова Т. А., Ольшанская Л. Н., Валиев Р. Ш. Культивирование и использование микроводорослей Chlorella и высших водных растений ряска Lemna. Саратов : ИЦ «Наука», 2017. 125 с.</mixed-citation><mixed-citation xml:lang="en">Politaeva N. A., Smyatskaya Yu. A., Kuznetsova T. A., Olshanskaya L. N., Valiev R. S. Kul'tivirovanie i ispol'zovanie mikrovodoroslei Chlorella i vysshikh vodnykh rastenii riaska Lemna [Cultivation and Use of Microalgae Chlorella and Higher Aquatic Plants Duckweed Lemna]. Saratov, ITs “Nauka” Publ., 2017. 125 p. (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Смятская Ю. А., Политаева Н. А., Собгайда В. С. Фотобиореакторы для культивирования микроводоросли Chlorella sorokiniana // Вестник Технологического университета. 2018. № 2. С. 224 – 227.</mixed-citation><mixed-citation xml:lang="en">Smyatskaya Y. A., Politaeva N. A., Sobgaida V. S. Photobioreactors for cultivating microalgae Chlorella sorokiniana. Bulletin of the Technological University, 2018, no. 2, pp. 224–227 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Субботина Ю. М., Смирнова И. Р., Кутковский К. А. Теоретические и методологические подходы к очистке сточных вод компонентами водной экосистемы // Вестник Алтайского государственного аграрного университета. 2015. № 5 (127). С. 99 – 106.</mixed-citation><mixed-citation xml:lang="en">Subbotina Yu. M., Smirnova I. R., Kutkovskiy K. A. Theoretical and methodological approaches to sewage treatment by the elements of aquatic ecosystem. Bulletin of Altai State Agricultural University, 2015, no. 5 (127), pp. 99–106 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Щербаков П. Н. Влияние избытка органического и неорганического углерода на поглощение ортофосфата клетками Chlorella vulgaris // Материалы международного молодежного научного форума «Ломоносов-2017». М. : МАКС Пресс, 2017. С. 1 – 2.</mixed-citation><mixed-citation xml:lang="en">Shcherbakov P. N. Influence of excess of organic and inorganic carbon on the absorption of orthophosphate by Chlorella vulgaris cells. International Youth Scientific Forum “Lomonosov2017”. Moscow, MAKS Press, 2017, pp. 1–2 (in Russian).</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Akhtar N., Iqbal M., Zafar S. I., Iqbal J. Biosorption characteristics of unicellular green alga Chlorella sorokiniana immobilized in loofa sponge for removal of Cr(III) // Journal of Environmental Sciences. 2008. Vol. 20, iss. 2. P. 231 – 239.</mixed-citation><mixed-citation xml:lang="en">Akhtar N., Iqbal M., Zafar S. I., Iqbal J. Biosorption characteristics of unicellular green alga Chlorella sorokiniana immobilized in loofa sponge for removal of Cr(III). Journal of Environmental Sciences, 2008, vol. 20, iss. 2, pp. 231–239.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Ardila L., Godoy R., Montenegro L. Sorption capacity measurement of Chlorella vulgaris and Scenedesmus acutus to remove chromium from tannery waste water // IOP Conference. Ser.: Earth and Environmental Science. 2017. Vol. 83. Article number 012031.</mixed-citation><mixed-citation xml:lang="en">Ardila L., Godoy R., Montenegro L. Sorption capacity measurement of Chlorella vulgaris and Scenedesmus acutus to remove chromium from tannery waste water. IOP Conference, Ser. Earth and Environmental Science, 2017, vol. 83, article number 012031.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Moheimani N. R., McHenry M. P., de Boer K., Bahri P. A. Biomass and Biofuels from Microalgae : Advances in Engineering and Biology. New York ; Dordrecht ; London : Springer International Publishing, 2015. 373 p.</mixed-citation><mixed-citation xml:lang="en">Moheimani N. R., McHenry M. P., de Boer K., Bahri P. A. Biomass and Biofuels from Microalgae: Advances in Engineering and Biology. New York, Dordrecht, London, Springer International Publishing, 2015. 373 p.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Politaeva N. A., Kuznetsova T. A., Smyatskaya Y. A., Trukhina E. V., Atamanyuk I. Energy production from Chlorella algae biomass under St. Petersburg climatic conditions // Chemical and Petroleum Engineering. 2018. Vol. 53, № 11. P. 801 – 805. https://doi.org/10.1007/s10556-018-0425-z</mixed-citation><mixed-citation xml:lang="en">Politaeva N. A., Kuznetsova T. A., Smyatskaya Y. A., Trukhina E. V., Atamanyuk I. Energy production from Chlorella algae biomass under St. Petersburg climatic conditions. Chemical and Petroleum Engineering, 2018, vol. 53, no. 11, pp. 801–805. https://doi.org/10.1007/s10556-018- 0425-z</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Shebanova A., Ismagulova T., Solovchenko A., Baulina O., Lobakova E., Ivanova A., Moiseenko A., Shaitan K., Polshakov V., Nedbal L., Gorelova O. Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy // Protoplasma. 2017. Vol. 254, iss. 3. P. 1323 – 1340.</mixed-citation><mixed-citation xml:lang="en">Shebanova A., Ismagulova T., Solovchenko A., Baulina O., Lobakova E., Ivanova A., Moiseenko A., Shaitan K., Polshakov V., Nedbal L., Gorelova O. Versatility of the green microalga cell vacuole function as revealed by analytical transmission electron microscopy. Protoplasma, 2017, vol. 254, iss. 3, pp. 1323–1340.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Smyatskaya Yu. A., Fazullina A. A., Politaeva N. A., Zhazhkov V. V., Pavlushkina Yu. E., Dolbnya I. V. The use and utilization of chitosan sorbents – The residual biomass of microalgae Chlorella sorokiniana // Ecology and Industry of Russia. 2019. Vol. 23, № 9. P. 18 – 23. https://doi.org/10.18412/1816-0395-2019-9-18-23</mixed-citation><mixed-citation xml:lang="en">Smyatskaya Yu. A., Fazullina A. A., Politaeva N. A., Zhazhkov V. V., Pavlushkina Yu. E., Dolbnya I. V. The use and utilization of chitosan sorbents – The residual biomass of microalgae Chlorella sorokiniana. Ecology and Industry of Russia, 2019, vol. 23, no. 9, pp. 18–23. https://doi.org/10.18412/1816-0395-2019-9-18-23</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Zhou W., Cheng Y., Li Y., Wan Y., Liu Y., Lin X., Ruan R. Novel fungal pelletization-assisted technology for algae harvesting and wastewater treatment // Applied Biochemistry and Biotechnology. 2012. Vol. 167, iss. 2. P. 214 – 228.</mixed-citation><mixed-citation xml:lang="en">Zhou W., Cheng Y., Li Y., Wan Y., Liu Y., Lin X., Ruan R. Novel fungal pelletizationassisted technology for algae harvesting and wastewater treatment. Applied Biochemistry and Biotechnology, 2012, vol. 167, iss. 2, pp. 214–228.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
